Bacterial Unknown Lab Report
Purpose: The purpose of this experiment is to demonstrate the comprehension of the lab techniques used throughout the semester and apply those methods in determining the identification of unknown number fourteen. Also, an in-depth explanation of the tests performed as well as the practices used, will enable in the assistance of clarifying any uncertainty that may occur.
Test 1: Results of Gram Positive Unknown
|Gram Stain||Crystal Violet, Gram’s Iodine, Ethyl Alcohol, Safranin||Cocci clusters, non-motile, catalase positive, factitive anaerobe, Grape like||Positive (+)||Gram Positive Clusters|
|Catalase||Hydrogen Peroxide||Bubbling, fizzing||Positive (+)||Gram positive bacteria has catalase enzyme|
|Coagulase||Slide Coagulase||No change||Negative (-)||Does no react with coagulase|
|Urea||Urea Broth Tube, Urea Slant||No color change; remained yellow||Negative (-)||Gram positive bacteria unable to produce urease|
1.) When the Gram Staining experiment took place, a clean glass slide with a small drop of H20 was first obtained. Then, with a sterile inoculating loop, the unknown bacterium was smeared onto the surface of the glass slide. After the unknown was spread on the slide, a Bunsen burner was then used to heat fix the bacterium to the surface of the slide. Next, a microscope, with the assistance of immersion oil, was then used to observe and classify the bacterium. The results we small “grape like” cluster of Gram positive bacteria. The observation can be seen below (e.g., Figure 1) as the results from the aforementioned experiment.
(Ex. Figure 1)
2.) When preforming the Catalase experiment a clean glass slide whipped with water was obtained, and then dried with bibulous paper and finally air-dried. Next, a drop of hydrogen peroxide was placed in the center of the slide. Then, with a sterilized inoculating loop, the test bacteria were transferred to the drop on the glass slide. The results can be observed in (Figure 2).
(Ex. Figure 2)
3.) In the next experiment, a clean glass slide was divided into two sides using a black marker. The two small drops of distilled H2O were situated on both divided slides of the slide. Next, a small amount of the unknown bacterium was gathered using a sterilized inoculating loop and then placed into the mixture. The results inconclusive as shown in (Figure 3).
(Ex. Figure 3)
4.) When undergoing the urea test, a sterile urea test tube was obtained. Then, after getting a sterilized inoculation loop, the urea broth was inoculated with the unknown culture. Next, the inoculate was placed into an incubator for twenty-four to forty-eight hours at thirty-seven degrees Celsius. After the incubation period was over, a generous amount of the unknown organism was gathered and streaked in a zig-zag fashion onto the slanted medium. After being incubated for forty-eight hours at thirty-seven degrees Celsius the results were negative as seen in (Figure 4)
(Ex. Figure 4)